Research Assistant

Employment Types:
Toronto, ON, Canada
Posted on:

The research assistant will execute the cell culture operations of Inteligex with direct oversight of at least one of its experimental programs. This position includes technically diverse bench work as well as the training of research staff in techniques and equipment use. The ideal applicant has a technical background in genome engineering, with a passion for working in a fast-paced environment to solve practical challenges and devise new methodologies.

What you’ll do • Assuring the quality and integrity of all data collection and documentation under GDocP guidelines and protocols • Accurately document experimental records and charts • Follows written standard operating procedures (SOP) • Expansion and cryopreservation of pluripotent stem cells in compliance with written SOPs • Generation of neural and glial lineages from pluripotent stem cells • Developing mutant and variant cell lines using CRISPR. • Performance of flow cytometry and cell sorting • Performance of routine imaging and image documentation of cells • RNA and DNA isolation and preparation for genomic analyses • Daily management of the cell culture lab as needed • Execute and help develop cell-based assays to support target characterization and optimization or cultures • Assisting in the training and supervision of junior laboratory staff • Assuring compliance with all relevant lab safety and biosafety regulations • Manage laboratory resources, ordering reagents and other lab supplies as needed (receipt and organization of incoming lab supplies) What we’re looking for • BSc or MS in neuroscience, cell biology, or biotechnology • Extensive hands-on expertise in pluripotent stem cell biology and stem cell culture techniques • 2-3+ years of post-graduate academic or industry experience in neural and glial cell biology • Significant expertise in and understanding of stem cell biology • Significant experience with immunochemistry, image analysis, and confocal microscopy • Excellent interpersonal, organizational, and independent problem-solving skills • Proficiency with standard cytometry and imaging software (e.g., FACSDiva, FlowJo, ImageJ, etc.) • Knowledge of laboratory safety including biohazard handling and disposal techniques, and the ability to ensure laboratory compliance with required safety regulations. • Must be an effective communicator both verbally and in writing What will separate you from the crowd • Experience in deriving both neuronal and glial cells from pluripotent stem cells • Experience in making clonal lines of pluripotent stems cells • Experience in genome editing techniques (i.e. CRISPR) • Neuroscience background

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